Which high-sensitivity test is preferred for detecting hepatitis B surface antigen?

The preferred high-sensitivity test for detecting hepatitis B surface antigen (HBsAg) is the ELISA (Enzyme-Linked Immunosorbent Assay). This assay is widely used due to its ability to provide qualitative and quantitative results for HBsAg detection. ELISA tests are highly sensitive and specific, making them effective for early diagnosis of hepatitis B infection.

The ELISA method utilizes antibodies specific to the hepatitis B surface antigen, allowing for the detection of even low levels of the antigen in a sample. This makes it particularly valuable in clinical settings where early detection can influence treatment decisions and management of the disease.

In contrast, while a Western blot is a reliable method for confirming the presence of antibodies or proteins, it is not typically used as a first-line test for HBsAg due to its complexity, longer processing time, and lower throughput. The immune compliance test is not a standard or commonly recognized method for detecting hepatitis B surface antigen. The PCR (Polymerase Chain Reaction) test, although sensitive and capable of detecting the viral genetic material, is primarily used to quantify viral load rather than specifically for the direct detection of HBsAg. Therefore, ELISA remains the most effective and practical choice for initial screening of hepatitis B surface